Journal:

Article Title: Cytokine response to vitamin E supplementation is dependent on pre-supplementation cytokine levels

doi:

Figure Lengend Snippet: Description of SNPs, primers and probes

Article Snippet: IL-1β was detected using mouse anti-human IL-1β MAb and biotinylated anti-human IL-1β Ab (R&D Systems, Minneapolis, MN).

Techniques:

Journal:

Article Title: Cytokine response to vitamin E supplementation is dependent on pre-supplementation cytokine levels

doi:

Figure Lengend Snippet: Genotype frequencies

Article Snippet: IL-1β was detected using mouse anti-human IL-1β MAb and biotinylated anti-human IL-1β Ab (R&D Systems, Minneapolis, MN).

Techniques:

Journal:

Article Title: Cytokine response to vitamin E supplementation is dependent on pre-supplementation cytokine levels

doi:

Figure Lengend Snippet: The effect of vitamin E supplementation on cytokine production depends on baseline cytokine production. Cytokine production was measured from whole blood at the beginning and end of a one year vitamin E supplementation in the elderly. TNF-α, IL-1β, and IL-6 was measured from whole blood elicited for 24 hours with lipopolysacchride (LPS; 1.0 µg/mL). IFN-γ was measured from whole blood elicited for 48 hours with phytohemagluttinin (PHA; 20 µg/mL) or concalavinA (ConA; 40 µg/mL). Cytokines production was corrected for the number of monocytes and lymphocytes in the blood (pg/ 106 lymphocyte and monocyte). P values (P) for the interaction are unadjusted.

Article Snippet: IL-1β was detected using mouse anti-human IL-1β MAb and biotinylated anti-human IL-1β Ab (R&D Systems, Minneapolis, MN).

Techniques: